From Mittwoch (1964b).īarr bodies are most commonly situated at the periphery of the nucleus. Sex chromatin adjacent to nucleolus in fibroblast of female origin (thionin, × 2200). Here the demonstration of Barr bodies presents considerable difficulties.įIG. In some mammals, particularly rodents, the chromocenters are much more prominent. In cases where a small percentage of Barr bodies have been reported in male nuclei, this is almost certainly due to nonsex-specific chromocenters, which were sufficiently similar to Barr bodies to have been mistaken for them. In cells of human origin, such nonspecific chromocenters are generally smaller than Barr bodies and have a less well-defined outline. Interphase nuclei are not always quite homogeneous, and they may contain a number of more or less darkly staining bodies, which are known as chromocenters. The average size has been estimated as 0.7 × 1.2 μ, both in nuclei of buccal mucosa and in sections of several human tissues ( K. The size of a Barr body is about 1 μ in diameter. It is present in a large proportion of nuclei of female origin and absent in male nuclei. A Barr body is a small, well-defined body which stains intensely with nuclear dyes ( Figs.
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